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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was identified to associate with, and become associated with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and plays a crucial role in advertising mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually described. Two additional splice variants ended up noted but in-depth nucleotide sequence data isn't but available.
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Blocking peptides are peptides that bind precisely for the goal antibody and block antibody binding. These peptide generally incorporates the epitope identified through the antibody.
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This manual outlines more doable optimization of mobile and immunoassay parameters to guarantee the absolute best outcomes are...
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This guide outlines further possible optimization of cellular and immunoassay parameters to guarantee the absolute best results are...
Antibodies bound to the blocking peptide no more bind into the epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Target Data Cyclin E belongs to your extremely conserved cyclin family members, whose members are characterized by a extraordinary periodicity in protein abundance from the cell cycle. Cyclins perform as regulators of CDK kinases. Different cyclins show unique expression and degradation patterns which add on the temporal coordination of each mitotic party. Cyclin E kinds a complex with and capabilities being a regulatory subunit of CDK2, whose activity is necessary for cell cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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